Radioimmunological determination of serum 3 beta-hydroxy-5-cholenoic acid in normal subjects and patients with liver disease.
A radioimmunoassay for the determination of 3 beta-hydroxy-5-cholenoic acid in human serum has been developed, using 3 beta-hydroxy-5-cholenoyl-thyroglobulin as immunogen Americano Cup and 3 beta-hydroxy-5-cholenoylglycyl-125I histamine as radioactive ligand.The association constant was 6.3 X 10(8) l/mol.Cross reactivity with other bile acids of hu